تقييم تفاعل البلمرة المتسلسل والاختبارات المصلية والمزرعة لتشخيص عدوى الجهاز التنفسي ببكتيريا الكلاميديا نيمونيا

Evaluation of PCR, Serology and Culture for Diagnosis of Chlamydia Pneumoniae in Respiratory Fathia Muftah 

                Chlamydophila (Chlamydia) pneumonia, is a gram-negative obligate intracellular bacterium and it is a wide spread respiratory pathogen.

                In this study, 112 specimens were examined in patients with a diagnosis of community acquired pneumoniae.

                Each of these samples was interpretted by four techniques. these techniques were cell culture, IMF, ELISA and PCR.

                In cell culture 46 (41.07%) were positive, while 66 (58.93%) were negative.

                Indirect IMF 45 (40.18%) were positive, while 67 (59.82%) were negative.

                Then ELISA 80 (71.42%) were positive, while 32 (28.57%) were negative.

                Then PCR 65 (58.04%) were positive, while 47 (41.96%) were negative. 

                Our study showed that males were more susceptible to Chlamydial infection than females.

                Also, the results represented that the most susceptible group to infection was the group C (30-86 years old).

                The sensitivity of different methods compared in this study showed that ELISA is the most sensitive technique (80/112) were positive.

                However, PCR (65/112) were positive, IMF (45/112) were positive and culture (45/112) were positive, show less sensitivity."